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Mito RealThiol (MitoRT) provides ratiometric fluorescent readout for quantification of mitochondrial GSH in live mammalian cells

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The GSH-dependent detoxifying machinery is constitutively maintained in choroid plexuses of Nrf2 knockout animals, but dimethylfumarate-driven induction of choroidal glutathione-S-transferase activity is strongly reduced To assess whether DMF-mediated induction of GST activity depends strictly on the activation of the Nrf2 signaling pathway, we used Nrf2 knockout rats